The transport of [3-3H]oleanolic acid and its monoglycosides to isolated vacuoles of protoplasts from Calendula officinalis leaves
1992
Janiszowska, W. | Szakiel, A.
Intact vacuoles isolated from C. officinalis leaf protoplasts were supplied with [3-(3)H]oleanolic acid, its 3-O-monoglucoside and 3-O-monoglucuronide. It was found that both monoglycosides were transported into vacuolar space whereas free oleanolic acid only bound with the tonoplast. The effects of pH of the medium and the presence of ATP on transport of monoglycosides, as well as the competition between oleanolic acid glycosides in their transport to isolated vacuoles, were studied. The results indicate that different mechanisms underlie the transport of both series of oleanolic acid glycosides to the vacuoles.
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