The embryogenic capacity of seven cucumber (Cucumis sativus L.) cultivars were examined by tissue culture of cotyledon, young first-leaf, and internode explants. Somatic embryogenesis frequencies differed significantly among the tested cultivars, and 'Fushinarimidori' produced the highest number of embryos from either cotyledons of young first leaves. Cotyledon- and first-leaf-derived calluses produced more embryos than calluses from internodes. Somatic embryos were induced from 'Aonaga F1, internodes with relatively high sucrose levels (6% and 9%) in the initiation medium, the frequency of embryogenic callus formation from 'Fushinarimidori' cotyledon explants was > 90%. The highest yield of somatic embryos occurred in cultures initiated with high sucrose levels (9% or 12%), although 12% sucrose inhibited callus formation and growth. Somatic embryos germinated in a basal liquid medium supplemented with 0.5% activated charcoal, and they developed into well-shaped, healthy plantlets on semisolid medium with 1% sucrose.
显示更多 [+] 显示较少 [-]
AGRIS - 国际农业科技情报系统
Share
HortScience
卷
29
期
8
页码
906
- 909
ISSN
0018-5345
其它主题
Embryogenic callus; Embryo (plant)
语言
英语
注释
2019-12-05
类型
Text; Journal Article
2024-02-28
MODS