Epidemiology of Tilletia indica teliospores in regulated wheat fields in Texas
2005
Stein, J.M. | Maples, H.W. | Rush, C.M.
To examine the epidemiology of Tilletia indica teliospores in naturally infested soils from wheat fields in both Karnal bunt-regulated regions in Texas, soil was grid sampled from fields that were bunted-kernel positive for Karnal bunt in 1997, 2001, both years, or never. Aliquots of soil from each point were pooled, and teliospores were extracted using a size-selective sieving-sucrose centrifugation method. Teliospores were enumerated microscopically, and low quantities (< 8 per 25 g of soil) were identified in 14 of 15 fields sampled from the regulated regions of Texas, including fields that have never tested positive for bunted kernels, indicating a widespread distribution. No teliospores were isolated from the single field examined outside of the regulated regions. The percent clay was significantly, negatively correlated with the baseline teliospore number and the estimated (extrapolated) number of teliospores per sample, indicating a potential impact of soil composition on teliospore survival. The latter factor was also significantly, positively correlated to the number of times a field had tested positive.
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