Histopathological alterations induced by subacute fipronil toxicity in mice and its amelioration by combination of a-tocopherol and ascorbic acid
2014
Badgujar, Prarabdh C. | Pawar, N.N. | Telang, A.G. | Kurade, N. P. | Chandratre, G.A. | Kadave, Mayur
Swiss albino female mice were randomly divided into four equal groups comprising 6 animals in each. Group 1 was administered vehicle, corn oil and served as control. Group 2 mice received fipronil @ 10 mg/kg body weight; mice in the group 3 were administered with combination of a-tocopherol and ascorbic acid, each @ 100 mg/kg body weight. Mice of group 4 were treated with a-tocopherol and ascorbic acid, each @ 100 mg/kg body weight, 2 hours before administration of fipronil @ 10 mg/kg body weight. Dosage were administered orally daily by gavage for consecutive 28 days. Mice were sacrificed at the end of 28 days to study gross and histopathological alterations. Grossly, liver of mice treated with fipronil was fragile with change of consistency and showed scattered pale foci. In histopathology, liver of mice treated with fipronil showed severe degenerative changes in heptocytes with presence of large number of necrotic foci, infiltration of mononuclear cells and congestion. In kidney, severe degenerative changes along with necrosis of tubular lining cells and congestion were evident in the fipronil treated mice. Severe emphysema and rupture of alveoli along with congestion was observed in the lung of mice treated with fipronil. Testis also showed depopulation of germs cells and thickening in basal layer of seminiferous tubules in fipronil treated mice. Co-administration of combination of these two vitamins against fipronil restored gross and histological changes in these organs. This study indicated that, subacute exposure of fipronil @ 10 mg/kg bw results in heaptotoxicity and cytotoxicity. Combined administration of antioxidant vitmains exerted protective action and ameliorated histological architecture of major organs.
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