Vigorous responsiveness of dwarf gene Rht14 to exogenous GA3 evaluated on important morphological and agronomic traits in durum wheat
2020
Duan, Shan | Zhao, Zhangchen | Qiao, Yue | Cui, Chunge | Condon, Anthony G. | Ma, Yukun | Hu, Yin‐Gang
Rht14, was considered as a GA‐responsive dwarf gene in durum wheat (Triticum durum L.). The object of this paper is to understand the responsiveness of Rht14 after applying exogenous GA₃ on important morphological and agronomic traits, and its potential application in wheat breeding. In this study, the F₂:₃ lines, F₃:₄ lines and F₄:₅ lines derived from the reciprocal crosses between the Castelporziano (CP), the donor of Rht14, and tall cultivar Langdon (L) were used to evaluate the effects of Rht14 with exogenous GA₃ application on crucial traits. After application of GA₃, the plant height of the dwarf lines was more significantly increased by an average of 51.21% and 52.27% than that of the tall lines by 14.70% and 15.16% of the two populations, respectively, while their diameter and thickness of internodes were remarkably reduced, and their flag leaf area, SPAD value and net photosynthetic rate were markedly decreased, compared with the controls. After application of GA₃, the spikelet number spike⁻¹, grain number spike⁻¹, and 1000‐kernel weight of the dwarf lines notably increased, while no significant differences were observed on the seed length, width and thickness. Exogenous GA₃ significantly increased the coleoptile length, whereas notably reduced the max root length and total root length of the dwarf lines. Significant increases of crude protein, wet gluten, sedimentation value, stability time and development time were observed in the dwarf lines treated with GA₃. This study indicated the dwarf gene Rht14 was a GA‐deficient gene and application GA₃ could rescue some negative effects by Rht14.
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