Molecular mapping and characterization of an RGA locus RGAPtokin1-2(171) in chickpea
2002
Rajesh, P.N. | Tekeoglu, M. | Gupta, V.S. | Ranjekar, P.K. | Muehlbauer, F.J.
Resistance gene analog polymorphism (RGAP) is a targeted homology based method, which has been used in different crops to identify tightly linked markers for disease resistance genes and also to enrich the map with a different class of markers. In chickpea, using the RGA primers, which are designed based on the conserved motifs present in characterized R-genes, Bulk Segregant Analysis (BSA) was performed on a resistant bulk and a susceptible bulk along with parents for ascochyta blight resistance. Of all available RGAs and their 48 different combinations, only one RGA showed polymorphismduring BSA. This marker was evaluated in an F7:8 population of 142 RILs from an interspecific cross of C.arietinum (FLIP 84-92C) x C. reticulatum (PI 599072) and was mapped to Cicer linkage map. The genomic location of chickpea RGA was compared with the locations of mapped chickpea R-genes. This is the first RGA marker mapped to chickpea linkage map.
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