Efficacy of washing with hydrogen peroxide followed by aerosolized antimicrobials as a novel sanitizing process to inactivate Escherichia coli O157:H7 on baby spinach
2012
Huang, Yaoxin | Ye, Mu | Chen, Haiqiang
Aerosolization was investigated as a potential way to apply allyl isothiocyanate (AIT), hydrogen peroxide (H₂O₂), acetic acid (AA) and lactic acid (LA) on fresh baby spinach to control Escherichia coli O157:H7 during refrigeration storage. In this study, baby spinach leaves were dip-inoculated with E. coli O157:H7 to a level of 6 log CFU/g and stored at 4°C for 24h before treatment. Antimicrobials were atomized into fog-like micro-particles by an ultrasonic nebulizer and routed into a jar and a scale-up model system where samples were treated. Samples were stored at 4°C for up to 10days before the survival of the cells was determined. A 2-min treatment with 5% AIT resulted in a >5-log reduction of E. coli O157:H7 on spinach after 2days refrigeration regardless if the samples were pre-washed or not; however, this treatment impaired the sensory quality of leaves. Addition of LA to AIT improved the antimicrobial efficacy of AIT. In the jar system, washing with 3% H₂O₂ followed by a 2-min treatment of 2.5% LA+1% AIT or 2.5% LA+2% AIT reduced E. coli O157:H7 population by 4.7 and >5 log CFU/g, respectively, after 10days refrigeration. In the scale-up system, up to 4-log reduction of bacterial population was achieved for the same treatments without causing noticeable adverse effect on the appearance of leaves. Thus, this study demonstrates the potential of aerosolized AIT+LA as a new post-washing intervention strategy to control E. coli O157:H7 on baby spinach during refrigeration storage.
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