A Rapid Method Based on Butanol Extraction for Selecting High-Lysine Maize (Zea mays L.)
1971
Fromberg, H. K. | Christ, W. | Pollmer, W. G.
Whole kernel samples from 48 selfed lines (S₂, S₃ of maize (Zea mays L.) were obtained from crosses involving normal and the mutant opaque-2. Kernels from these samples were classified as translucent, semitranslucent, and opaque in appearance. All were treated with watersaturated n-butanol at room temperature. The extraction resulted in losses of nitrogen ranging from 0 to 40%. The nitrogen compounds extracted were nearly free of lysine. The proportion of butanol-insoluble nitrogen to total nitrogen was closely correlated with lysine content of the protein, with an r value of .817. Similarly, the insoluble nitrogen was closely correlated (r = .847) with the lysine content on a whole-kernel basis. Since the method requires only the simple procedures of nitrogen determination and butanol extraction, it should be very suitable for screening a large number of maize samples for high lysine.
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