Characterization of Fenoxaprop-P-Ethyl–Resistant Junglerice (Echinochloa colona) from Mississippi
2016
Wright, Alice A. | Nandula, Vijay K. | Grier, Logan | Showmaker, Kurt C. | Bond, Jason A. | Peterson, Daniel G. | Ray, Jeffery D. | Shaw, David R.
A population of junglerice from Sunflower County, MS, exhibited resistance to fenoxaprop-P-ethyl. An 11-fold difference in ED₅₀ (the effective dose needed to reduce growth by 50%) values was observed when comparing the resistant population (249 g ae ha⁻¹) with susceptible plants (20 g ae ha⁻¹) collected from a different field. The resistant population was controlled by clethodim and sethoxydim at the field rate. Sequencing of the acetyl coenzyme A carboxylase, which encodes the enzyme targeted by fenoxaprop-P-ethyl, did not reveal the presence of any known resistance-conferring point mutations. An enzyme assay confirmed that the acetyl coenzyme A carboxylase in the resistant population is herbicide sensitive. Further investigations with two cytochrome P450 inhibitors, malathion and piperonyl butoxide, and a glutathione-S-transferase inhibitor, 4-chloro-7-nitrobenzofurazan, did not indicate involvement of any metabolic enzymes inhibited by these compounds. The absence of a known target-site point mutation and the sensitivity of the ACCase enzyme to herbicide show that fenoxaprop-P-ethyl resistance in this population is due to a non–target-site mechanism or mechanisms.Nomenclature: Clethodim; fenoxaprop-P-ethyl; sethoxydim; junglerice, Echinochloa colona Link. ECHCO
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