Marker-assisted introgression of the Or gene for enhancing β-carotene content in Indian cauliflower
2018
Kalia, P. | Muthukumar, P. | Soi, S.
Among micronutrients, vitamin A is essential for vision; its deficiency leads to night blindness, which is the most prominent malnutrition problem in India. Since cauliflower is grown and consumed extensively all year round in India, but is deficient in vitamin A, it was identified as a preferred crop for biofortification in order to address a malnutrition problem in the country. We report on the introgression of the β-carotene (pro-vitamin A)-enhancing Or gene from donor line EC625883 into Indian cauliflower 'Pusa Sharad' (DC 309) and the parents of 'Pusa Hybrid-2' (CC-35 and DC 18-19). The sequence-characterized amplified region (SCAR) marker SA4 was utilized for foreground selection in the 'Pusa Sharad' (DC 309) × EC625883 BC1F1 cross. A total of 1013 PCR-based markers were used for a polymorphic survey among parents, with 6.81% being polymorphic between 'Pusa Sharad' and EC625883, 6.91% between CC-35 and EC625883, and 6.71% between DC 18-19 and EC625883. Background analysis showed a maximum recovery of the recurrent parent genome (RPG) to the level of 72%. The β-carotene content of BC1F1 plants of the dark-orange category was 2.03-14.64 µg g‑1 (DC 309 × EC625883), 7.03-15.99 µg g‑1 (CC-35 × EC625883) and 4.52-18.60 µg g‑1 (DC 18-19 × EC625883). The combination of high RPG and β-carotene content was recorded in two plants, 18-19-1-7-7 (18.60 µg g‑1) and 18-19-1-3-6 (17.94 µg g‑1). Promising lines showing more than 10 µg g‑1 β-carotene in BC1F1 were advanced to BC1F2 and BC2F1.
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