Effect of the Ethoxy Analog of Rhizobitoxine on Nodulation of Soybeans
1980
Devine, T. E. | Breithaupt, B. H.
A common property of Rhizobium japonicum strains that are able to nodulate the nodulation restrictive soybean [Glycine max (L.) Merr.] genotype rj₁ rj₁ is the ability to produce rhizobitoxine (2-amino-3-hydroxypropoxyvinylglycine). The ethoxy analog of rhizobitoxine (aminoethoxyvinylglycine) was tested for potential to modify the nodulating ability of strains of Rhizobium japonicum (Kirchner) Buchanan with Clark rj₁rj₁ soybeans. The analog was added to broth cultures of Rhizobium at concentrations of 0, 5 ✕ lO⁻¹⁰, 5 ✕ 10⁻⁸, 5 ✕ 10⁻⁶, 5 ✕ 10⁻⁶ and 5 ✕ 10⁻⁴ M, 30 hours before being used to inoculate seeds planted in Leonard jars. The analog did not enhance nodulation at any of the concentrations tested. Genetic modification of the rhizobial microsymbiont appears to be a more promising approach for obtaining Rhizobium strains capable of successfully nodulating the rj₁ rj₁ genotype.
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