An LRR receptor kinase involved in perception of a peptide plant hormone, phytosulfokine
2002
Matsubayashi, Y. | Ogawa, M. | Morita, A. | Sakagami, Y.
The sulfated peptide phytosulfokine (PSK) is an intercellular signal that plays a key role in cellular dedifferentiation and proliferation in plants. Using ligand-based affinity chromatography, we purified a 120-kilodalton membrane protein, specifically interacting with PSK, from carrot microsomal fractions. The corresponding complementary DNA encodes a 1021-amino acid receptor kinase that contains extracellular leucine-rich repeats, a single transmembrane domain, and a cytoplasmic kinase domain. Overexpression of this receptor kinase in carrot cells caused enhanced callus growth in response to PSK and a substantial increase in the number of triticum-labeled PSK binding sites, suggesting that PSK and this receptor kinase act as a ligand-receptor pair.
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