Lipoxygenase activity in menhaden gill tissue and its effect on odor of n-3 fatty acid ester concentrates
1994
Grun, I.U. | Barbeau, W.E.
Menhaden oil is highly susceptible to oxidation due to a high concentration of n-3 polyunsaturated fatty acids, leading to objectionable off-odors and off flavors. The purpose of this study was to investigate if the enzyme lipoxygenase is present in menhaden gill tissue and if its activity on n-3 fatty acid esters can be detected by a sensory panel. Lipoxygenase activity was found in the crude enzyme extract from menhaden gill tissue. The enzyme exhibited its highest activity at pH 9-10, and was inhibited by esculetin. Panelists judged the smell of n-3 fatty acid ethyl ester concentrates that were incubated with the enzyme extract to be significantly stronger than that of untreated controls. It can be concluded that menhaden gill tissue contains an active lipoxygenase which possibly contributes to the peroxidation of menhaden oil.
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