Development of seventeen polymorphic microsatellite loci in Spinibarbus sinensis (Bleeker)
2012
Xiong, Meihua | Shi, Fang | Shao, Ke | Liang, Yinquan | Liu, Yugang | Lai, Hongzhi | Wu, Chengbin | Zhu, Bin
We developed 17 polymorphic microsatellite loci in Spinibarbus sinensis. All markers were obtained from partial genomic DNA libraries enriched for tetranucleotide repeats and characterized in 32 unrelated individuals from one putative population in Wujiang River. The number of alleles ranged from 3 to 17, with an average of 7.471 per locus. The expected and observed heterozygosity values ranged from 0.647 to 0.910 and 0.333 to 1.000, respectively. Three loci showed significant deviation from Hardy–Weinberg Equilibrium. No significant lingkage disequilibrium was found. These polymorphic loci described for S. sinensis will be valuable as genetic markers for use in breeding, genetic management and conservation studies.
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