Biosynthesis of Vitamin B₆ in Rhizobium: In Vitro Synthesis of Pyridoxine from 1-Deoxy-D-xylulose and 4-Hydroxy-L-threonine
2002
TAZOE, Masaaki | ICHIKAWA, Keiko | Hoshino, Tatsuo
Pyridoxine (vitamin B₆) in Rhizobium is synthesized from 1-deoxy-D-xylulose and 4-hydroxy-L-threonine. To define the pathway enzymatically, we established an enzyme reaction system with a crude enzyme solution of R. meliloti IFO14782. The enzyme reaction system required NAD⁺, NADP⁺, and ATP as coenzymes, and differed from the E. coli enzyme reaction system comprising PdxA and PdxJ proteins, which requires only NAD⁺ for formation of pyridoxine 5′-phosphate from 1-deoxy-D-xylulose 5-phosphate and 4-(phosphohydroxy)-L-threonine.
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书目信息
出版者
Japan Society for Bioscience, Biotechnology, and Agrochemistry
其它主题
Enzymatic reactions; Vitamin b6in vitro synthesis; 1-deoxy-d-xylulose; 4-hydroxy-l-threonine
语言
英语
注释
Nal-ap-2-clean
类型
Journal Article; Text
2024-02-28
MODS