Identification of catalytically active groups of pea (Pisum sativum L.) β-glucosidase

Ershova, A. N.; Barkalova, O. N.

Identification of catalytically active groups of pea (Pisum sativum L.) β-glucosidase

2011

Ershova, A. N. | Barkalova, O. N.

Functional groups of cytoplasmic pea β-glucosidase pretreated to an electrophoretically homogeneous state were identified. Data on the pH dependence of the enzyme activity, calculated heat of ionization, photoinactivation of the enzyme in the presence of methylene blue, and inactivation of the enzyme with diethyl pyrocarbonate suggest that the catalytic site of β-glucosidase contains the carboxyl group of glutamic or aspartic acids and the imidazole group of histidine.

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