Assessing leaf nitrogen content and leaf mass per unit area of wheat in the field throughout plant cycle with a portable spectrometer
2013
Ecarnot, Martin | Compan, Frédéric | Roumet, Pierre
Leaf nitrogen content (LNC) and leaf mass per unit area (LMA) were assessed by near-infrared spectroscopy (NIRS) on fresh and dried plants of durum wheat (Triticum turgidum ssp). Individual leaves were scanned with a portable spectrometer and reference analyses of LNC and LMA were then carried out. Partial least squares (PLS) regression was used for calibration and cross-validation. LNC was accurately predicted for both fresh and dry leaves whatever the phenologic stage (correlation coefficient of calibration R2cal ranging from 0.932 to 0.958, standard error of cross-validation SECV ranging from 0.215 to 0.320% (dry matter)). LMA was predicted with R2cal=0.942 and SECV=4.84gm−2. The combination of these two calibrations made it possible to predict leaf nitrogen per unit area (R2cross-validation=0.94, SECV=0.248gNm−2) and provides a relevant and non-destructive tool for following the dynamics of three major leaf parameters.
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