Martelella alba sp. nov., isolated from mangrove rhizosphere soil within the Beibu Gulf
2021
Li, Mi | Gao, Chenghai | Feng, Yuyao | Liu, Kai | Cao, Pei | Liu, Yonghong | Yi, Xiangxi
Strain BGMRC 2036ᵀ was isolated from rhizosphere soil of Bruguiear gymnorrhiza collected from the Beibu Gulf of China. Optimum growth occurred at 28 °C, pH 7.0, and under the conditions of 3–5% (w/v) NaCl. The phylogenetic comparisons of 16S rRNA gene sequences displayed that strain BGMRC 2036ᵀ was closely related to Martelella limonii NBRC109441ᵀ (96.6% sequence similarity), M. mediterranea CGMCC 1.12224ᵀ (96.5%), M. lutilitoris GH2-6ᵀ (96.5%), M. radicis BM5-7ᵀ (96.2%), and M. mangrove BM9-1ᵀ (95.9%), M. suaedae NBRC109440ᵀ (95.8%). The phylogenomic tree based on the up-to-date bacterial core gene set indicated that the strain BGMRC 2036ᵀ form a clade formed with members of the genera Martelella. The major polar lipids include phosphatidylmethylethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphotidylinositol, two unidentified phospholipids, and three unidentified ninhydrin positive phospholipids. The major respiratory quinone is Q-10, which is similar to those of genera Martelella. The main cellular fatty acids are C₁₈:₁ ω7c, C₁₆:₀, and C₁₂:₀ aldehyde. Genome sequencing revealed a genome size of 4.99 Mbp and a G + C content of 62.3 mol%. Pairwise comparison of the genomes of the new strain BGMRC 2036ᵀ and the three reference strains M. endophytica YC 6887ᵀ, M. mediterranea CGMCC 1.12224ᵀ, and M. mangrovi USBA-857 indicated that gANI value was lower than 81% and a digital DNA–DNA hybridization value was lower than 27%. The strain BGMRC 2036ᵀ possessed genes putatively encoding riboflavin synthesis and flavodoxin A polyphasic taxonomic study suggested that strain BGMRC 2036ᵀ represented a novel species belonging to the genus Martelella, and it was named Martelella alba sp. nov. The type strain is BGMRC 2036ᵀ (=KCTC 52121ᵀ =NBRC 111908ᵀ).
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