FTIR-ATR infrared spectroscopy for the detection of ochratoxin A in dried vine fruit
2007
Galvis-Sanchez, A.C. | Barros, A. | Delgadillo, I.
A method of screening sultanas for ochratoxin A (OTA) contamination, using mid-infrared spectroscopy/Golden Gate single-reflection ATR (attenuated total reflection), is described. The main spectral characteristics of sultanas from different sources were identified in a preliminary acquisition and spectral analysis study. Principal component analysis (PCA) showed that samples of various origins had different spectral characteristics, especially in water content and the fingerprint region. A lack of reproducibility was observed in the spectra acquired on different days. However, spectral repeatability was greatly improved when water activity of the sample was set at 0.62. A calibration curve of OTA was constructed in the range 10-40 microgram OTA kg(-1). Samples with OTA levels higher than 20 microgram kg(-1) were separated from samples contaminated with a lower concentration (10 microgram OTA kg(-1)) and from uncontaminated samples. The reported methodology is a reliable and simple technique for screening dried vine fruit for OTA.
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