Conversion of biomass hydrolysates and other substrates to ethanol and other chemicals by Lactobacillus buchneri
2009
Liu, S. | Bischoff, K.M. | Hughes, S.R. | Leathers, T.D. | Price, N.P. | Qureshi, N. | Rich, J.O.
A Lactobacillus buchneri strain NRRL B-30929 can convert xylose and glucose into ethanol and chemicals. The aims of the study were to survey three strains (NRRL B-30929, NRRL 1837 and DSM 5987) for fermenting 17 single substrates and to exam NRRL B-30929 for fermenting mixed substrates from biomass hydrolysates. Mixed acid fermentation was observed for all three L. buchneri strains using various carbohydrates; the only exception was uridine which yielded lactate, acetate and uracil. Only B-30929 is capable of utilizing cellobiose, a desired trait in a potential biocatalyst for biomass conversion. Flask fermentation indicated that the B-30929 strain can use all the sugars released from pretreated hydrolysates, and producing 1·98-2·35 g l⁻¹ ethanol from corn stover hydrolysates and 2·92-3·01 g l⁻¹ ethanol from wheat straw hydrolysates when supplemented with either 0·25x MRS plus 1% corn steep liquor or 0·5x MRS. The L. buchneri NRRL B-30929 can utilize mixed sugars in corn stover and wheat straw hydrolysates for ethanol and other chemical production. These results are valuable for future research in engineering L. buchneri NRRL B-30929 for fermentative production of ethanol and chemicals from biomass.
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