Molecular detection of Bovine Papilloma Virus types associated with papillomatous lesions in large animals and toxico-pathological effects of Pteriscreticain rats

In the present investigation, the prevalence and pathology of Papillomavirus induced cutaneous neoplasms in equines, the status and pathology of cutaneous and mucosal Bovine Papilloma Virus (BPV) associated neoplasms in cattle and buffaloes and experimental evaluation of toxicopathological effects of Pteriscretica, in laboratory rats were studied. These spontaneous cases were assessed by PCR, histopathology and immunohistochemistry.A total of 19 suspected cutaneous neoplasmswere recorded in present investigation in equines. The major neoplasm was squamous cell carcinoma (8 cases) followed by equine sarcoid (4 cases). The squamous cell carcinoma (SCC) was mostly observed on eyes followed by genetalia (penis/prepuce), palate and perineal region. The squamous cell carcinoma (3/8) and equine sarcoid (4/4) showed positivity for BPV-1 and -2 DNA by PCR. The immunohistochemical expression of Connexin 26 marker was detected intracytoplasmically in most of the cases of SCC. A total of 290 urinary bladders from non enzootic areas for EBH- like Chennai, Thrissur and Bareilly revealed very low prevalence of benign neoplasms (fibromas-2 and leiomyoma-1) but epithelial abnormalities viz hyperplasia and inflammatory conditionslike lymphocytic cystitis predominated. These benign neoplasms and different pathological conditions of urinary bladder showed positivityfor BPV-1/-2 by PCR. Few archival urinary bladders (Transitional cell carcinoma-4, Haemangiosarcoma-1, Haemangioma- 1 and Adenoma-1)from EBH cases were also included in the study. The benign neoplasms, archival urinary bladder tumours, chronic cystitis and hyperplasia cases were subjected to expression of H-ras oncogene by immunohistochemistry. A total of 3/4 of transitional cell carcinoma and 1 haemangioma were found H-ras positive but rest of the casesi.e., benign tumours and inflammatory conditions failed to show any H-rasimmunoexpression.MMP7 expression could not be detected in both cutaneous neoplasms and urinary bladder tumours. A total of 63 bubaline female genetaliastudied showed only inflammatory lesions but cases of neoplasms were not diagnosed.However, a clinical case of vulvar squamous cell carcinoma in a cow was recorded. Selected (28) inflammatory genital lesions were screened for BPV DNA but all samples were found negative. The phylogenetic analysis of the sequences obtained in present study showed close homology with the previous published Indian sequences of BPV-1 and -2.