Strand specific PCR amplification of low copy number DNA
1992
Meyerhans, A. | Vartanian, J.P. | Wain-Hobson, S.
A method for the amplification of a single DNA strand at low copy number is described. It is a wholly PCR based approach which involves an initial linear amplification of the target using a tagged strand specific primer. This is followed by classical PCR amplification of the progeny using a pair of primers, one specific for the sequence tagged onto the 5' end of the first round primer, the second specific for the target sequence. Given the protocol used the ratio of the two strands in the final amplification product was 50:1.
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书目信息
Nucleic acids research
卷
20
期
3
页码
521
- 523
ISSN
0305-1048
其它主题
Single-stranded; Molecular; Dna amplification; Base sequence; Biochemical techniques; Oligodeoxyribonucleotides; Molecular sequence data
语言
英语
类型
Journal Article; Text
2024-02-28
MODS