Lipid production from a mixture of sugarcane top hydrolysate and biodiesel-derived crude glycerol by the oleaginous red yeast, Rhodosporidiobolus fluvialis
2018
Poontawee, Rujiralai | Yongmanitchai, Wichien | Limtong, Savitree
Lipid was produced by Rhodosporidiobolus fluvialis DMKU-SP314 from a mixture of low-cost substrates: sugarcane top hydrolysate (STH) and biodiesel-derived crude glycerol. The optimized lipid production medium for shaking flask cultivation contained STH supplemented with 59g/L crude glycerol, 0.21g/L soybean powder, 0.9g/L (NH4)2SO4, 0.4g/L KH2PO4, 2.0g/L MgSO4·7H2O and pH 6.1 (C/N ratio 60), while the optimal cultivation temperature was 28°C. Batch cultivation in a 2L stirred-tank fermenter at an agitation speed of 300rpm and an aeration rate of 2vvm achieved the maximum lipid quantity of 18.2g/L, which was 75.0% of dry biomass (24.3g/L biomass), after 240h of cultivation. These represent a 5.4-fold and 1.7-fold improvement in the lipid concentration and lipid content, respectively, compared to non-optimized shaking flask cultivation. Under optimal conditions, the lipid produced had a high monounsaturated fatty acid content (36.6% of oleic acid), which is suitable for biodiesel production. Thus, R. fluvialis DMKU-SP314 is capable of producing large quantities of lipid from low-cost raw materials in a process that can be readily scaled up for industrial production.
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