Isolation and screening for novel bioactive molecules from endophytes associated with Arisaema erubescens (wall.) shott
2021
Rathore, Akanksha | Singh, Brajeshwar | Raina, Diksha | Kour, Sneahpreet | Dutta, Upma | Singh, A.K. | Rai, Gyanendra Kumar
Arisaema erubescens is one of the medicinal herbs found abundantly in the Himalayan region. Healthy plant samples of Arisaema erubescens were collected from Patnitop situated at an elevation of 2, 065 metres (6, 640 ft), with33° 05′15.60"N and 75° 19′49.91"E coordinates of District Udhampur in Jammu Division. Total nine bacterial and eight fungal endophytes were isolated from plant samples. Bacterial endophytes were identified on the basis of Morphological & Biochemical behaviour. Later on promising cultures were subjected to 16S rRNA tools for species identification. Fungal endophytes were identified on the basis of morphological & microscopic characteristics and most of them were of Aspergillus genus. Endophytes were screened for their antibiotic production potential against human pathogens Pseudomonas aeruginosa (MTCC741), Escherichia coli (MTCC1697), Staphylococcus aureus (MTCC96) and Salmonella typhimurium (MTCC98J. Out of nine bacterial isolates only MB-19 & MB-25 has shown significant anti-microbial activities against Salmonella typhimurium and Escherichia coli respectively. Potent endophytes were processed for identification of active metabolites at Natural Product Lab, IIIM (CSIR) Jammu. Bacterial extracts were subjected to bioassay for confirmation of presence of active metabolites in extracts. MB-19 & MB-25 has retained antimicrobial activity. Methanol and chloroform extracts of MB-19 and MB-25 were subjected to HPLC. 13 peaks were recorded in chloroform extract and 11 peaks in methanol extract for the culture.no.MB-19 and in MB-25, 18 peaks in chloroform extract and 6 peaks of methanol extract were recorded. This study concluded that Arisema erubescens retains ample biodiversity within which might be the reason for its medicinal values.
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