Target‐site mutation associated with cross‐resistance to ALS‐inhibiting herbicides in late watergrass (Echinochloa oryzicola Vasing.)
2013
Kaloumenos, Nikolaos S | Chatzilazaridou, Sofia L | Mylona, Photini V | Polidoros, Alexios N | Eleftherohorinos, Ilias G
BACKGROUND: Studies were carried out to elucidate the mechanism of resistance to ALS‐inhibiting herbicides in 29 Echinochloa accessions from water‐seeded rice fields of northern Greece and to discriminate the Echinochloa species. RESULTS: Two E. oryzicola accessions were found to be cross‐resistant to penoxsulam, bispyribac‐sodium, imazamox, foramsulfuron, nicosulfuron and rimsulfuron, whereas all accessions were susceptible (S) to profoxydim. Sequencing of the ALS gene revealed that resistant (R) accessions had a Trp574Leu mutation, which was also confirmed by TspRI endonuclease digestion. Use of cpDNA sequence comparison analysis of Echinochloa species discriminated successfully E. crus‐galli and E. oryzicola accessions. CONCLUSION: This is the first report of Echinochloa oryzicola cross‐resistance to ALS‐inhibiting herbicides as a result of Trp574Leu mutation. The cpDNA sequence comparison analysis is a reliable tool for discrimination of conventionally classified E. crus‐galli and E. oryzicola accessions. © 2012 Society of Chemical Industry
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