Effects of H2O2 on growth, metabolic activity and membrane integrity in three strains of Microcystis aeruginosa

The application of hydrogen peroxide (H₂O₂) as a management tool to control Microcystis blooms has become increasingly popular due to its short lifetime and targeted action. H₂O₂ increases intracellular reactive oxygen species resulting in oxidative stress and subsequently cell death. H₂O₂ is naturally produced in freshwater bodies as a result of photocatalytic reactions between dissolved organic carbon and sunlight. Previously, some studies have suggested that this environmental source of H₂O₂ selectively targets for toxigenic cyanobacteria strains in the genus Microcystis. Also, past studies only focused on the morphological and biochemical changes of H₂O₂-induced cell death in Microcystis with little information available on the effects of different H₂O₂ concentrations on growth, esterase activity and membrane integrity. Therefore, this study investigated the effects of non-lethal (40–4000 nM) concentrations on percentage cell death; with a focus on sub-lethal (50 μM) and lethal (275 μM; 500 μM) doses of H₂O₂ on growth, cells showing esterase activity and membrane integrity. The non-lethal dose experiment was part of a preliminary study. Results showed a dose- and time-dependent relationship in all three Microcystis strains post H₂O₂ treatment. H₂O₂ resulted in a significant increase in intracellular reactive oxygen species, decreased chlorophyll a content, decreased growth rate and esterase activity. Interestingly, at sub-lethal (50 μM H₂O₂ treatment), percentage of dead cells in microcystin-producing strains was significantly higher (p < 0.05) than that in non-microcystin-producing strains at 72 h. These findings further cement our understanding of the influence of H₂O₂ on different strains of Microcystis and its impact on membrane integrity and metabolic physiology: important to future toxic bloom control programmes.