Visualization of EXGT-mediated molecular grafting activity by means of a fluorescent-labeled xyloglucan oligomer
1999
Ito, H. | Nishitani, K.
A fluoresceinyl xyloglucan heptasaccharide was synthesized and used as a probe to visualize the activity of the cell wall to incorporate xyloglucan fragments into the cellulose/xyloglucan framework in suspension-cultured tobacco cells. The activity was significantly reduced in transformant cells in which the expression of endoxyloglucan transferase (EXGT) was severely suppressed by over-expression of the antisense EXGT-N1 mRNA. These results indicate the involvement of EXGT in the construction of the cellulose/xyloglucan framework.
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书目信息
出版者
Elsevier Ltd
其它主题
Genbank/ab017025; Molecular sequence data; Endoxyloglucan transferase; Cell suspension culture; Genbank/d86730; Xyloglucans; Cell wall components; Hexosyltransferases; Nucleotide sequences
语言
英语
注释
2019-12-04
类型
Journal Article; Text
2024-02-28
MODS