Transcriptional analysis of sex pheromone biosynthesis signal genes in Bombyx mori
2012
An, Shi‐Heng | Du, Meng‐Fang | Su, Li‐Juan | Yin, Xin‐Ming
Six sex pheromone synthesis signal genes, including acyl coenzyme A (acyl‐CoA) desaturase (desat1), fatty acyl reductase (FAR), pheromone biosynthesis activating neuropeptide receptor (PBANR), fatty acid transport protein (FATP), acyl‐CoA binding protein (ACBP) and store‐operated channel protein (Orai1A), were studied for their transcriptional regulations. The expression profiles of these transcripts at different developmental stages (from –96 to 48 h) revealed that the genes are expressed in an age‐dependent manner. The transcripts of these genes continued to increase despite decapitation, and compared with normally developmental females, decapitation significantly inhibited their expression. Further experiments with a methoprene, a juvenile hormone (JH) analogue, challenge showed that JH was not a key inhibiting factor in the expression of these genes, and mating was found to significantly inhibit the expression of these marker genes. Altogether, the results provide a reference for understanding the mechanism of sex pheromone synthesis.
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