LC Separation and Quantification of Tocopheryl Polyethylene Glycol Succinate and Tocopheryl Acid Succinate in TPGS Reaction Mixture
2009
Wang, Jingyuan | Su, Baogen | Su, Yun | Yang, Yiwen | Ren, Qilong
An analytical method was proposed for the determination of d-α-tocopheryl polyethylene glycol 1,000 succinate (TPGS 1000) and d-α-tocopheryl acid succinate (TAS) in TPGS reaction mixture with a simple and inexpensive reversed-phase liquid chromatographic method. The LC separation was carried out on a C18 analytical column with UV detection at 284 nm using acetonitrile and water (90:10, v/v) with 0.5% acetic acid (pH 3.73) at a flow rate of 1.5 mL min⁻¹. The average recoveries of TPGS were between 95.41 and 96.82%, while those of TAS were between 96.69 and 102.39%. Within-day and day-to-day relative standard deviations of TPGS were less than 0.23 and 0.29%, while those of TAS were less than 0.17 and 0.27%. The content of TPGS was determined in terms of the hydrolyzed α-tocopherol. The alkaline hydrolysis process demonstrated high α-tocopherol recoveries between 98.78 and 103.05%. This method has been applied successfully to the accurate determination of TAS and TPGS in highly complex reaction mixture to monitor the reaction process progress.
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