Antioxidant properties and free radicals scavenging activities of pomegranate (Punica granatum L.) peels: An in-vitro study
2022
Sihag, Sonam | Pal, Ajay | Ravikant, | Saharan, Vinod
Fruit peels often contain more bioactive substances than the edible component of the fruit. As a result, the current study was designed to use a variety of in-vitro assays to investigate the antioxidant capacity of phenolic compounds found in pomegranate (Punica granatum L.) peel. The IC₅₀ values for DPPH free radical scavenging activity, ABTS assay, and lipid peroxidation inhibition activity were as low as 0.1, whereas the same for hydroxyl radical scavenging activity was 0.6 μg/ml. The nitric oxide scavenging activity had the lowest IC₅₀ value (0.02 μg/ml). At a concentration of 0.21 μg/ml, phenolics scavenged ∼60% of DPPH radicals, while at a phenolic content of 98.9 μg/ml, the extract chelated 60% of the iron metal. The RP₀.₅AU value of total reducing power was 0.37 μg/ml. Total antioxidant activity (AO₀.₅AU value of 4.24 μg/ml) was significantly lower than the standard (8.42 g/ml). At a dose of 0.09 μg/ml, phenolics reduced lipid peroxidation by 53% in an egg homogenate model. Gel electrophoresis indicated that the extracts with phenolic content of 20 and 40 μg virtually protected the BSA against AAPH-induced oxidation. Overall, various experiments showed that pomegranate peel extract has significant antioxidant activity and supports its use as a source of natural antioxidants to combat autoxidation-induced pathologies or diseases and use in the health food/nutraceutical/pharmaceutical industry for a variety of applications.
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