Characterization and expression analysis of six MADS-box genes in maize (Zea mays L.)
2012
Zhang, Zhongbao | Li, Huiyong | Zhang, Dengfeng | Liu, Yinghui | Fu, Jing | Shi, Yunsu | Song, Yanchun | Wang, Tianyu | Li, Yu
MADS-box genes encode a family of transcription factors, which control diverse developmental processes in flowering plants, with organs ranging from roots, flowers and fruits. In this study, six maize cDNAs encoding MADS-box proteins were isolated. BLASTX searches and phylogenetic analysis indicated that the six MADS-box genes belonging to the AGL2-like clade. qRT-PCR analysis revealed that these genes had differential expression patterns in different organs in maize. The results of yeast one-hybrid system indicated that the protein ZMM3-1, ZMM3-2, ZMM6, ZMM7-L, ZMM8-L and ZMM14-L had transcriptional activation activity. Subcellular localization of ZMM7-L demonstrated that the fluorescence of ZMM7-L-GFP was mainly detected in the nuclei of onion epidermal cells. qRT-PCR analysis for expression pattern of ZMM7-L showed that the gene was up-regulated by abiotic stresses and down-regulated by exogenous ABA. The germination rates of over-expression transgenic lines were lower than that of the wild type on medium with 150mM NaCl, 350mM mannitol. These results indicated that ZMM7-L might be a negative transcription factor responsive to abiotic stresses.
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