Production of cellulases and hemicellulases by Penicillium echinulatum grown on pretreated sugar cane bagasse and wheat bran in solid-state fermentation
2007
Camassola, M. | Dillon, A.J.P.
To evaluate the solid-state fermentation (SSF) production of cellulase and hemicellulases (xylanases), by Penicillium echinulatum 9A02S1, in experiments carried out with different concentrations of the pretreated sugar cane bagasse (PSCB) and wheat bran (WB). This study reports the production of xylanolytic and cellulolytic enzymes by P. echinulatum 9A02S1 using a cheap medium containing PSCB and WB under SSF. The highest amounts of filter paper activity (FPA) could be measured on mixtures of PSCB and WB (32·89 ± 1·90 U gdm⁻¹). The highest β-glucosidase activity was 58·95 ± 2·58 U gdm⁻¹ on the fourth day. The highest activity for endoglucanases was 282·36 ± 1·23 U gdm⁻¹ on the fourth day, and for xylanases the activity was around 10 U gdm⁻¹ from the second to the fourth day. The present work has established the potential of P. echinulatum for FPA, endoglucanase, β-glucosidase and xylanase productions in SSF, indicating that WB may be partially substituted by PSCB. The incorporation of cheap sources, such as sugar cane bagasse, into media for the production of lignocellulose enzymes should help decrease the production costs of enzymatic complexes that can hydrolyse lignocellulose residues for the formation of fermented syrups, thus contributing to the economic production of bioethanol.
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