Effect of explants source on shoot proliferation and adventitious regeneration in 10 Buddleia cultivars
2009
Phelan, Sinead | Hunter, A. | Douglas, G.C.
Viable shoot cultures and weaned plants were obtained from cultured apical meristems with 10 Buddleia cultivars giving viabilities of 32-72%. The number of shoots produced, the micropropagation rate and the root number produced in vitro was higher in meristem derived shoots compared to those derived from shoot-tips. The subsequent growth rate of meristem derived plants, in the greenhouse, was also greater. The number of roots produced by conventional cuttings collected from meristem derived plants was significantly higher than in cuttings which were collected from plants derived from shoot-tips or from the original stock plants. Endogenous bacteria were not detected in either shoot cultures derived from meristems or in 10-week-old weaned plants derived from meristems whereas those derived from shoot-tips showed the presence of endogenous bacteria when sterilized explants were cultured on nutrient agar or on tryptic soy broth. Factors affecting adventitious bud and shoot production in leaf and internode explants was determined for 'Lochinch', 'Border Beauty', 'Ile de France' and 'Pink Delight' using meristem derived shoot cultures. Adventitious shoots appeared after 4 weeks of culture, in both types of explant when cultured on MS supplemented with 0.5-5.0μM TDZ. The highest percentage regeneration was achieved from bisected internode explants cultured on 0.5μM TDZ, with 93-100% regeneration among the cultivars whereas BA was less effective. The best response was obtained using 5.0μM TDZ which gave over 10-11 shoots per explant in all bisected explants for all cultivars.
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