Integration of single-molecule detection with endonuclease IV-assisted signal amplification for sensitive DNA methylation assay

Zhang, Yan; Hu, Jinping; Zou, Xiaoran; Ma, Fei; Qiu, Jian-Ge; Zhang, Chun-yang

Integration of single-molecule detection with endonuclease IV-assisted signal amplification for sensitive DNA methylation assay

2021

We demonstrate the development of a new fluorescent biosensor for sensitive DNA methylation assay by integrating single-molecule detection with endo IV-assisted signal amplification. This biosensor possesses the characteristics of good selectivity and high sensitivity with a detection limit of 7.3 × 10⁻¹⁷ M. It can distinguish as low as 0.01% methylation level, and can analyze genomic DNA methylation even in a single cancer cell.

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