Analysis of maltooligosaccharides in honey samples by ultra-performance liquid chromatography coupled with evaporative light scattering detection
2014
Zhou, Jinhui | Qi, Yitao | Ritho, Joan | Duan, Lili | Wu, Liming | Diao, Qingyun | Li, Yi | Zhao, Jing
We report the use of ultra performance liquid chromatography (UPLC) separation together with evaporative light scattering detection (ELSD) to effectively distinguish honey adulterated with maltooligosaccharides. UPLC separation utilizing a mobile phase with pure water and acetonitrile containing 0.2% triethylamine, resulted in enhanced separation and a running time of less than 6min. Also, only a simple liquid–liquid extraction was required for the sample pretreatment. This UPLC–ELSD method provides limits of quantifications (LOQs) from 0.30 to 0.60mg/g for the six maltooligosaccharides (maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose and maltoheptaose) ranging from 15 to 2000μg/mL with excellent linearity(R2≥0.9914). This optimized method also offers a satisfactory intra-day precision of 2.4%–9.4%, inter-day precision of 5.5%–12.1% and accuracy of −7.7%–+11.4% at each level. We successfully applied this system for detecting maltooligosaccharide contamination in 290 honey samples collected from apiaries, supermarkets and traditional agri-product markets. Concentrations of the six maltooligosaccharides varied from undetectable to 14.1mg/mL. The lowest quantifiable concentration that could be detected by this established method was 0.132mg/mL. This study therefore demonstrates the reliability and potential use of the UPLC–ELSD technique in scrutinizing the authenticity of honey in the food processing industry.
显示更多 [+] 显示较少 [-]