Effects of ammonium treatment on the protein and chlorophyll contents and the number of ribosomes in Chlamydomonas reinhardtii cells
2012
Smolov, A. P. | Semenova, G. A. | Minakova, N. Yu | Butanaev, A. M. | Shirshikova, G. N.
The effects of exogenous ammonium on total protein and chlorophyll contents and the number of ribosomes in Chlamydomonas reinhardtii (Sager) green alga cells were studied. The absence of ammonium from nutrient medium resulted in a twofold drop in the number of ribosomes in the cytoplasm. The chlorophyll content of the cells grown on ammonium-free nitrate-containing medium insignificantly increased. The increase in the ribosome number in the presence of exogenous ammonium was not accompanied by the elevation of total cellular protein and did not depend on the levels of mRNA of rps6, a small ribosome subunit protein, and the 18S rRNA. Possible reasons for the effect of exogenous ammonium during ribosome biogenesis are discussed.
显示更多 [+] 显示较少 [-]