AMPK activation ameliorates fine particulate matter-induced hepatic injury
2020
Song, Liying | Jiang, Shuo | Pan, Kun | Du, Xihao | Zeng, Xuejiao | Zhang, Jia | Zhou, Ji | Sun, Qinghua | Xie, Yuquan | Zhao, Jinzhuo
Both the epidemiological and animal experimental studies have reported the association between PM₂.₅ and respiratory, cardiovascular, and metabolic diseases. However, the study linking PM₂.₅ and hepatic injury is few, and the relative mechanism has not been fully elucidated. Thirty-two 6-week-old male C57BL/6 mice were exposed to filtered air (FA) or concentrated PM₂.₅ for 12 weeks using Shanghai Meteorological and Environmental Animal Exposure System (“Shanghai-METAS”), respectively. At week 11, the mice began to be treated with intraperitoneal injection of normal 0.9% saline or AMPK activator (AICAR). The mRNA levels of IL-6 and TNF-α, and protein expressions of AMPK, GLUT4, NF-κB, p38MAPK, ERK, and JNK in the liver and UCP-1 in brown adipose tissue (BAT) were measured. Meanwhile, histopathological examination both in the liver and BAT was performed to evaluate the histopathological changes. PM₂.₅ exposure induced steatosis, hepatocyte ballooning, lobular and portal inflammation in the liver, and the brown adipocyte swelling in BAT. The results found that PM mice displayed higher IL-6, TNF-α, NF-κB, and JNK expression and lower AMPK, GLUT4, and UCP-1 when compared with FA mice. The AICAR injection upregulated the expressions of GLUT4 in the liver of PM-AIC mice when compared with the PM mice. However, there were no significant effects of AICAR on histopathological condition. The current study showed that ambient PM₂.₅ exposure might induce the hepatic injury along with the lipid metabolism disorder in BAT. AMPK activation can ameliorate most of the harmful effects and might become the potential target for treating PM₂.₅-induced hepatic injury.
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