Induction of ovalbumin mRNA by ascorbic acid in primary cultures of tubular gland cells of the chicken oviduct
1995
Muramatsu, T. | Hiramatsu, H. | Okumura, J.I.
The present study was conducted to investigate the effects of aging and medium supplements on steroid-induced ovalbumin mRNA in primary cultures of tubular gland cells from the chicken oviduct. In experiment 1, the effect of aging was examined by comparing the responsiveness to administration of estrogen and corticosterone in cells derived from laying hens and estrogen-primed chicks. In experiment 2, the effect of supplementing a culture medium with various compounds on the responsiveness to the steroid treatment was examined. In experiment 3, the effect of supplementing with ascorbic acid was tested in the presence or absence of the steroid hormones. The results indicated that the oviduct cells from immature chicks had clearer induction of ovalbumin mRNA by the steroid treatment than did those from laying hens. Among medium supplements, ascorbate increased the steroid responsiveness to a great extent, and fetal calf serum had modest, but long lasting, induction of ovalbumin mRNA. The drastic induction of ovalbumin mRNA by ascorbic acid supplementation was exerted only when the steroid hormones were present in the medium, implying that the effect of ascorbic acid may be auxiliary in steroid-induced transcription of the ovalbumin gene.
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