Data in the activities of caspases and the levels of reactive oxygen species and cytochrome c in the •OH-induced fish erythrocytes treated with alanine, citrulline, proline and their combination
2016
Li, Huatao | Jiang, Weidan | Liu, Yang | Jiang, Jun | Zhang, Yongan | Wu, Pei | Zhao, Juan | Duan, Xudong | Zhou, Xiaoqiu | Feng, Lin
The present study explored the effects of alanine (Ala), citrulline (Cit), proline (Pro) and their combination (Ala₁₀Pro₄Cit₁) on the activities of caspases and levels of reactive oxygen species (ROS) and cytochrome c in hydroxyl radicals (•OH)-induced carp erythrocytes. The data displayed that •OH induced the increases in the activities of caspase−3, caspase−8 and caspase−9 and the levels of ROS and cytochrome c in carp erythrocytes. However, Ala, Cit, Pro and Ala₁₀Pro₄Cit₁ effectively suppressed the •OH-induced increases in the activities of caspase−3, caspase−8 and caspase−9 and the levels of ROS and cytochrome c in carp erythrocytes. Furthermore, the activities of caspase−3, caspase−8 and caspase−9 and the levels of ROS and cytochrome c were gradually decreased with increasing concentrations of Ala, Cit, Pro and Ala₁₀Pro₄Cit₁ (0.175−1.400mM) in the •OH-induced carp erythrocytes. These data demonstrated that the 50% inhibitory doses (ID₅₀) of Ala₁₀Pro₄Cit₁ on the activities of caspase−8, caspase−9 and caspase−3 and levels of ROS and cytochrome c were respectively estimated to be the minimum values among amino acids examined so far. The 5% inhibitory doses (ID₅) of Ala, Cit, Pro and Ala₁₀Pro₄Cit₁ on the activities of caspase−8, caspase−9 and caspase−3 and levels of ROS and cytochrome c were estimated to be at their physiological concentrations in mammalian. Our research article for further interpretation and discussion from these data in Li et al. (2016) [1].
显示更多 [+] 显示较少 [-]