Hepatic microsomal drug-metabolizing enzyme in aflatoxin B1-treated rats after feeding vegetable diet Amaranthus hybridus
1993
Mbiapo, F. | Moundipa, P.F.
The effect of vegetable diet on the drug-metabolizing enzymes after aflatoxin B1 treatment were studied in male rats for 30 d. The diets contained various levels of the leafy vegetable Amaranthus hybridus, 0 (Ah0), 200 (Ah200) or 600 (Ah600) g/kg, and the animals were given intraperitoneally injected aflatoxin B1 (either 0 or 0.2 mg/kg body weight) in propylene glycol every two days. After 10, 20, and 30 d, the animals were killed and their serum and hepatic microsomes prepared for the assay of enzymes. The group of rats fed diet containing 600 A. hybridus showed highest gain, whereas those fed diet without vegetable showed lowest weight gain. The cytochrome P-450 content of the liver, as well as the activity of aminoprine N-demethylase and that of aniline hydroxylase was high in the group fed Ah600. When rats were given aflatoxin B1, the level of cytochrome P-450 decreased in group fed diet Ah600, showing a significant diet X aflatoxin B1 interaction. For UDPglucuronyltransferase, rats fed on diet containing 200 or 600g A. Hybridus/kg diet showed the highest activity in this enzyme. After aflatoxin B1 treatment, group fed on diet Ah0 showed a significant increase in the activity of UDPglucuronyltransferase (186 percent) after 30 d. This was seen as being a sign of toxicity of aflatoxin B1 towards the rats fed on the above diet. This experiment emphasizes the ability of leafy vegetable A. hybridus in inducing hepatic liver drug-metabolizing enzymes through improvement of nutrient content of the diet.
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