Protective effect of Terminalia catappa leaf extracts against Saprolegniosis on angelfish eggs
2022
Meneses, Juliana Oliveira | da Silva, Izadora Cibely Alves | da Cunha, Ana Flávia Santos | dos Santos Cunha, Fernanda | Dias, Joel Artur Rodrigues | Abe, Higo Andrade | Paixão, Peterson Emmanuel Guimarães | da Costa Sousa, Natalino | do Couto, Márcia Valéria Silva | dos Santos Lima, Bruno | de Carvalho Neto, Antônio Guilherme | de Souza Araújo, Adriano Antunes | da Costa, Luiz Pereira | dos Santos, Francisco José | Cardoso, Juliana Cordeiro | Diniz, Leandro Eugenio Cardamone | Fujimoto, Rodrigo Yudi
The Saprolegniosis causes major reduction egg hatching rate by angelfish (Pterophyllum scalare); therefore, we investigated the effectiveness of two Terminalia catappa extracts (27 ℃ and 80 ℃) against S. parasitica on fish eggs. Mycelium growth was evaluated in vitro at 0, 2.5, 5.0 and 10 g/L for 96 h, in solid and liquid medium culture. In an in vivo assay, we tested lower concentrations of hot extract (25, 50 and 100 mg/L) to prevent infection by zoospores. The hot aqueous extract was the most effective, and the concentrations of 5 and 10 g/L demonstrated great efficiency in liquid medium. In vivo conditions, 50 and 100 mg/L of hot extract provided more viable eggs, greater percentage of total larvae and lower percentage of infected eggs. Thus, the hot extract should be used instead of the room‐temperature extract as a prophylactic measure, to prevent saprolegniosis in P. scalare eggs and improving the hatching rate.
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