AFLP analysis of genetic diversity within a jackfruit germplasm collection
2001
Schnell, R.J. | Olano, C.T. | Campbell, R.J. | Brown, J.S.
Twenty-six jackfruit (Artocarpus heterophyllus) accessions, and one each for an interspecific hybrid (A. heterophyllus × A. interger), champedak, and breadfruit (A. altilis), were analysed using amplified fragment length polymorphic (AFLP) markers to determine the degree of genetic diversity within the Fairchild Tropical Garden (FTG), Florida, USA germplasm collection. Of the 30 primer pairs evaluated, 12 were identified for collection screening based on the number and quality of polymorphic fragments. A total of 187 AFLP markers were scored using the 12 primer pairs, 92 (49.2%) being polymorphic. All accessions could be uniquely identified using the 12 primer pairs. Among the jackfruit accessions, similarity coefficients ranged from 0.567 to 0.950; the accessions also shared a large number of monomorphic fragments (54.9%). Cluster analysis and principal component analysis (PCA) grouped all of the jackfruit accessions with southeast Asian origins into one major cluster, with little bootstrap support for groupings within the cluster. Indian accessions were grouped in a different cluster, as the hybrid and the breadfruit accessions. The AFLP-marker-based analysis indicates that limited genetic diversity exists within this collection. These observations are in agreement with the phenotypic evaluation, suggesting that new accessions can be obtained from the centre of origin of species.
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