Screening of non starch polysaccharide degrading enzymes and enzyme combinations for their ability to degrade non starch polysaccharides of broiler diet by two stage in vitro digestion assay
2013
Narasimha, J. | Nagalakshmi, D. | Reddy, Y Ramana | Rao, S.T Viroji
The investigation was undertaken to evolve a suitable NSP enzyme combination with potential to improve the digestibility of corn and soybean meal based diets for broilers. Two stage in vitro NSP digestibility assay was carried to arrive at a suitable NSP enzyme combination comprising of xylanase, cellulase and β-D-glucanase with or without protease for corn-soybean meal based broiler diets. The degree of NSP digestibility was assayed by measuring the amount of total sugars released. Various concentrations of Non-starch Polysaccharide enzymes viz., xylanase (0-60000 IU/kg), cellulase (0–6400 IU/kg), β-D-glucanase (0–6400 IU/kg) and protease (0-64000 IU/kg) were screened by in vitro digestibility assay for broilers. From that three best concentrations of NSP enzymes were selected for each enzyme to formulate twenty seven (3x3x3) enzyme combinations. In case of protease five concentrations were selected. The in vitro sugars release for broiler diet was significantly higher (P<0.01) for concentrations of 5000, 7500 and 10000 xylanase IU/kg; 50, 100 and 400 cellulase IU/kg; 100, 200 and 400 β-D-glucanase IU/kg and 500, 1000, 2000, 4000 and 8000 protease IU/kg diet. Among the twenty seven enzyme combinations of xylanase, cellulase and β-D-glucanase, the combination comprising of xylanase-7500, cellulase-100 and β-D-glucanase-100 IU/kg and with or without 2000 IU protease/kg was selected for broiler diet. It can be concluded that In vitro NSP digestibility assay, simulating the gut conditions of poultry could be used as a simple and effective technique to evolve suitable NSP enzyme combination with most effective concentration of enzymes specifically for diets of broilers formulated with various feed ingredients and also to arrive at best effective dosage level.
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