High frequency of the potential pathogen Lactococcus garvieae in raw milk from Kosovo
2015
Mehmeti, Ibrahim | Muji, Skender | Diep, Dzung B. | Nes, Ingolf F.
The present study was undertaken to investigate the microbial quality of raw milk from dairy farms in Kosovo with emphasis on the potential pathogen Lactococcus garvieae. Based on selective media and growth profiles approximately 770 isolates were obtained from raw milk collected from 221 farms and 119 of these isolates were shown to belong to L. garviae based on 16S rRNA gene sequencing. The 119 L. garvieae isolates were derived from 43 farms. It also appeared that L. garviae was established as a persistent bacterium in these farms since it was found in most samples collected over a period of 6 months. The L. garvieae-containing farms were not geographically related as isolates of this bacterium were found across the country. Genetic diversity assessed by rep-PCR fingerprinting revealed 8 major groups and the largest group contained isolates from 13 farms. Isolates within the individual groups 1, 2, 3, 4, and 5 showed strong genetic relatedness while the isolates from the remaining groups 6, 7 and 8, did not give a clear picture of relatedness. Twenty one isolates showed weak alpha haemolytic activity and antibiotic resistance was demonstrated in 39 of the 43 isolates. Diversity in the fermentation profiles was also observed among the 43 L. garvieae isolates that could be divided into 5 separate fermentation groups.
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