Regeneration of garlic callus as affected by clonal variation, plant growth regulators and culture conditions over time

Myers, J.M.; Simon, P.W.

Regeneration of garlic callus as affected by clonal variation, plant growth regulators and culture conditions over time

1999

Myers, J.M. | Simon, P.W.

A long-term regeneration system for garlic (Allium sativum L.) clones of diverse origin was developed. Callus was initiated on a modified Gamborg's B-5 medium supplemented with 4.5 micromolar 2,4-D and maintained on the same basal medium with 4.7 micromolar picloram +0.49 micromolar 2iP. Regeneration potential of callus after 5, 12 and 16 months on maintenance medium was measured using several plant growth regulator treatments. The 1.4 micromolar picloram +13.3 micromolar BA treatment stimulated the highest rate of shoot production. Regeneration rate decreased as callus age increased, but healthy plantlets from callus cultures up to 16-months-old were produced for all clones. Regeneration of long-term garlic callus cultures could be useful for clonal propagation and transformation.

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