Partial characterization of a bacteriocin produced by Lactobacillus delbrueckii subsp. lactis UO004, and intestinal isolate with probiotic potential
2001
Boris, S. | Jimenez-Diaz, R. | Caso, J.L. | Barbes, C.
Aims: The partial characterization of a bacteriocin produced by a human Lactobacillus delbrueckii isolate with probiotic potential. Methods and Results: A bacterocin, UO004, was partially purified by cation exchange followed by a hydrophobic interaction column, biochemically characterized and the N-terminal region sequenced. Bacteriocin UO004 was found to be a hydrophobic, heat-stable polypeptide with an apparent molecular mass of 6 kDa. It was also stable and active over a wide pH range. Conclusions: The active compound was proteinaceous, heat-stable, and had a bactericidal (and bacteriolytic) mode of action on a limited number of micro-organisms. Such a narrow spectrum of activity is typical for bacteriocins produced by intestinal Lactobacillus. Significance and Impact of the Study: Bacteriocin UO004 from a probiotic strain is a new compound that does not share any homology with any other known lactic acid bacteria bacteriocin. Furthermore, Lact. delbrueckii is regarded as a suitable starter for the production of fermented milks.
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