Detection methods for <I>Mycobacterium avium </I> subsp <I>paratuberculosis</I> in milk and milk products: a review
2008
Slana, I. | Paolicchi, F. | Janstova, B. | Navratilova, P. | Pavlik, I.
<I>Mycobacterium avium</I> subsp. <I>paratuberculosis (MAP)</I> is the etiologic agent of paratuberculosis, a disease with considerable economic impact, principally on dairy cattle herds. Animals with paratuberculosis shed viable <I>MAP</I> especially in their milk, faeces and semen. <I>MAP</I> may have a role in the development of Crohn’s disease in humans via the consumption of contaminated milk and milk products. The current methods of milk pasteurization are not sufficient to kill all <I>MAP</I> cells present in milk and <I>MAP</I> has been cultured from raw or pasteurized milk and isolated from cheese. The purpose of the present study was to review the different methods used for detection of <I>MAP</I> in milk and milk products. We analyze the current methods for direct or non direct identification of <I>MAP</I> and culture and molecular biology methods that can be applied to milk and milk products.
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