After effects of treatment with visible light and uv radiation on amino acids, nucleic acids and protein patterns in broad bean seedlings
2011
Younis, M.E. | Hasaneen, M.N.A. | Abdel-Aziz, H.M.M.
The objective of the present study was to investigate the possible effects of direct exposure of broad bean seedlings to different light spectra (low visible light, high visible light, UV-A and UV-C), either alone or in combination, as stressful factors on amino acids, nucleic acids and protein patterns. High accumulation of both proline and glycine was operative under the above mentioned experimental conditions, in relation to controls. As for protein banding patterns, stressed broad bean seedlings showed the appearance of protein bands with molecular weights of 106, 102, 93, 91, 78, 72 and 28.5 kDa. On the other hand, disappearance of protein bands with molecular weights of 22.0, 18.4 and 16 kDa was operative. In addition, exposure of broad bean seedlings to the above mentioned stressful factors induced a significant decrease in the total amount and in the relative composition of the pool of nucleic acids (RNA and DNA), throughout the entire period of the experiment. The present results are discussed in relation to interference of the light radiations with the process of germination and with intermediary metabolism in broad bean seedlings.
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