Elicitation enhanced the production of bioactive compound and biomass accumulation in callus cultures of Glycyrrhiza glabra L
2022
Jaiswal, Nancy | Verma, Yashodhara | Misra, Pragati
Licorice (Glycyrrhiza glabra L.), Fabaceae, has a wide range of medicinal properties due to the metabolites found in the plant tissues. Callus cultures from Glycyrrhiza glabra were previously initiated in vitro using leaf as an explant and the current study was designed to examine the possible role of various elicitors (biotin, adenine sulphate, salicylic acid, putrescine, spermine, or spermidine) at different concentrations (25, 50, 75, and 100 mg L⁻¹) on the stimulation of biomass and their effects on the content of different metabolites such as total carbohydrates, protein, proline, phenol, alkaloids, flavonoids, and glycyrrhizin. Elicitation with different concentrations of elicitors increased the biomass and metabolite content in callus culture of G. glabra at different rates. The optimum concentration of adenine sulphate for maximum biomass accumulation (16.79 g flask⁻¹) was found to be at 50 mg L⁻¹ after incubation for 20 d. Adenine sulphate and putrescine were also found to stimulate the metabolite content to 3- to fourfold in the callus cultures as compared to that of control showing that metabolite content and antioxidant enzyme of Glycyrrhiza glabra can be enhanced by appropriate elicitation.
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