Solid-state fermentation for the production of biomass valorizing feruloyl esterase
2016
Gopalan, Nishant | Nampoothiri, Kesavan Madhavan | Szakacs, George | Parameswaran, Binod | Pandey, Ashok
Aspergillus niger ATCC 13497 has been used to produce feruloyl esterase type A enzyme. The process parameters were optimized to attain a maximum of 11984.1U/gds of enzyme activity within a period of 72h using destarched wheat bran as a substrate. The purified enzyme studied in the present work displays a pH optimum of 5, and a temperature optimum of 37°C. The enzyme has a size of 35kDa and exhibited moderate thermostability, with 41% of activity retained at 90°C. Ni +2 and Co+2 ions were found to have stimulatory activity on the enzyme with 66% and 78% increase in activity respectively. Cu+2 inhibited the activity by 88%. Statistical optimization enhanced the enzyme activity by 2.47 fold.
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